One of the biggest advantages we have in the trypanosome field is their genetic tractability. Tools such as inducible RNAi and over expression are well developed and have been used for many years. Although these tools are very powerful for studying individual genes, we are becoming increasingly interested in studying cohorts of genes and how they work together to impart function. Many labs are doing exciting work that combines RNAi libraries and sequencing technologies. To facilitate this, we have developed high throughput protein localisation tools. Recently published in Open Biology, we have optimised gene tagging to make it fast, reliable and scalable. We are expanding our repertoire of vectors to allow tagging with different colours and biochemical tags using different drug resistances. These are available on request.
I am also developing tools for functional analysis of proteins in a similar high throughput manner.