archived vector sequences

All versions of pPOT prior to v4  have been superseded. They are below for reference only.
Plasmid Name  version Genbank Seq Resistance markers Fusion tag Amplicon size
pPOTv4 blast-blast eGFP 4 genbank sequence N term: BLAST C term: BLAST eGFP N term: 1.85 kb C term: 2 kb
pPOTv4 blast-blast eYFP 4 genbank sequence N term: BLAST C term: BLAST eYFP N term: 1.85 kb C term: 2 kb
pPOTv4 blast-blast mNeonGreen 4 seq icon N term: BLAST C term: BLAST mNeonGreen N term: 1.85 kb C term: 2 kb
pPOTv4 blast-blast superfolder GFP 4 genbank sequence N term: BLAST C term: BLAST superfolder GFP N term: 1.85 kb C term: 2 kb
pPOTv4 blast-blast tdTomato 4 pPOTv4-blast-blast-tdTomato N term: BLAST C term: BLAST tdTomato N term: 2.5 kb C term: 2.7 kb
pPOTv4 blast-blast tagRFPt 4 pPOTv4-blast-blast-tagRFPt N term: BLAST C term: BLAST tagRFPt N term: 1.8 kb C term: 2 kb
pPOTv4 g418-g418 eGFP 4 pPOTv4-g418-g418-eGFP N term: G418 C term: G418 eGFP N term: 2.25 kb C term: 2.4 kb
pPOTv4 g418-g418 eYFP 4 pPOTv4-g418-g418-YFP N term: G418 C term: G418 eYFP N term: 2.25 kb C term: 2.4 kb
pPOTv4 g418-g418 superfolder GFP 4 pPOTv4-g418-g418-sfGFP N term: G418 C term: G418 superfolder GFP N term: 2.25 kb C term: 2.4 kb
pPOTv4 g418-g418 mNeonGreen 4 pPOTv4-g418-g418-mNeonGreen N term: G418 C term: G418 mNeonGreen N term: 2.25 kb C term: 2.4 kb
pPOTv4 g418-g418 tagRFPt 4 pPOTv4-g418-g418-tagRFPt N term: G418 C term: G418 tagRFPt N term: 2.25 kb C term: 2.4 kb
pPOTv4 g418-g418 tdTomato 4 pPOTv4-g418-g418-tdTomato N term: G418 C term: G418 tdTomato N term: 3 kb C term: 3.15 kb
pPOTv4 blast-blast Ty::HaloTag::Ty 4 pPOTv4 blast-blast Ty::HaloTag::Ty N term: BLAST C term: BLAST Ty::HaloTag::Ty N term: 2.1 kb C term: 1.9 kb
pPOTv4 blast-blast Ty(10) 4 pPOTv4-blast-blast-10TyGS N term: BLAST C term: BLAST Ty::GS(10) N term: 2.1 kb C term: 1.3 kb
pPOTv4 puro-puro eYFP 4 pPOTv4-blast-blast-YFP N term: PURO C term: PURO eYFP N term: 2 kb C term: 2.2 kb
  If you use these plasmids or any of their derivatives in your work, please cite:

Dean, S., Sunter, J., Wheeler, R. J., Hodkinson, I., Gluenz, E. Gull, K. A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids. Open Biol 5. 2015 (doi:10.1098/rsob.140197)

Please contact me if you have any problems with the protocol or any of the files.